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JoVE Journal
Immunology and Infection

This content is Open Access.

Pan-lyssavirus realtid RT-PCR for rabies diagnose
 
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Pan-lyssavirus realtid RT-PCR for rabies diagnose

Article DOI: 10.3791/59709-v 06:25 min July 10th, 2019
July 10th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

Denne real-time RT-PCR ved hjælp af dsDNA interkalerende farvestof er egnet til at diagnosticere lyssavirus infektioner. Metoden begynder med RNA ekstraheret fra rabies mistanke ante-mortem eller post mortem prøver, detaljer Master Mix forberedelse, RNA tilføjelse, opsætning af realtid maskine og korrekt fortolkning af resultater.

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Immunologi og infektion lyssavirus rabies real-time RT-PCR diagnose et-trins
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