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JoVE Journal Cancer Research
Vurdering Cell levedyktighet og død i 3D spheroid kulturer av kreftceller
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Vurdering Cell levedyktighet og død i 3D spheroid kulturer av kreftceller

Article DOI: 10.3791/59714-v • 10:33 min • June 16th, 2019
June 16th, 2019 •
1, 1, 1
1Section for Cell Biology and Physiology, Department of Biology, Faculty of Science, University of Copenhagen

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Her presenterer vi flere enkle metoder for å evaluere levedyktighet og død i 3D kreftcelle spheroids, som etterligner fysikalsk-kjemiske graderinger av in vivo svulster mye bedre enn 2D kulturen. Den spheroid modellen tillater derfor evaluering av effekten av kreft stoffet med forbedret oversettelse til in vivo-forhold.

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Kreftforskning Spheroids 3D cellekultur hengende fall celle levedyktighet brystkreft bukspyttkjertelkreft anti-kreft terapi narkotikabehandling kjemoterapi rekonstituert kjeller membran propidium iodide immunhistokjemi
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