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Journal / Genetics / In vivo genetisch scherm naar voren om nieuwe…
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JoVE Journal Genetics
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In vivo genetisch scherm naar voren om nieuwe neuroprotectieve genen in Drosophila melanogaster te identificeren

Article DOI: 10.3791/59720-v • 10:00 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1, 2, 1
1Department of Biological Sciences, University of Alabama, 2Department of Genetics, University of Wisconsin-Madison

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We presenteren een protocol met een voorwaartse genetische benadering van het scherm voor mutanten die neurodegeneratie in Drosophila melanogastertentoonstellen. Het bevat een klim test, histologie analyse, genmapping en DNA-sequencing om uiteindelijk nieuwe genen te identificeren die verband houden met het neuroprotectie proces.

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Genetica uitgave 149 Drosophila melanogaster neurodegeneratie forward genetische scherm klim test histologie GENMAPPING DNA-sequencing
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