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JoVE Journal Genetics
En Vivo Forward Genetic Screen para Identificar Nuevos Genes Neuroprotectores en Drosophila melanogaster
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En Vivo Forward Genetic Screen para Identificar Nuevos Genes Neuroprotectores en Drosophila melanogaster

Article DOI: 10.3791/59720-v • 10:00 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1, 1, 2, 2, 1, 2, 1
1Department of Biological Sciences, University of Alabama, 2Department of Genetics, University of Wisconsin-Madison

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Presentamos un protocolo utilizando un enfoque genético directo para la detección de mutantes que exhiben neurodegeneración en Drosophila melanogaster. Incorpora un ensayo de escalada, análisis histológico, mapeo genético y secuenciación de ADN para identificar en última instancia nuevos genes relacionados con el proceso de neuroprotección.

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Genética Número 149 Drosophila melanogaster,neurodegeneración pantalla genética hacia adelante ensayo de escalada histología mapeo genético secuenciación de ADN
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