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Journal / Cancer Research / Påvisning og overvåking av tumor assosiert…
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Cancer Research

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JoVE Journal Cancer Research
Påvisning og overvåking av tumor assosiert sirkulerende DNA i pasient Biofluids
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Påvisning og overvåking av tumor assosiert sirkulerende DNA i pasient Biofluids

Article DOI: 10.3791/59721-v • 06:53 min • June 8th, 2019
June 8th, 2019 •
1,2, 1, 1,2, 3, 1, 4,5, 1,2,5
1Center for Genetic Medicine, Children's National Health System, 2Institute for Biomedical Sciences, The George Washington University School of Medicine and Health Sciences, 3Dana-Farber Cancer Institute, 4Department of Neurology, University of California San Francisco, 5DIPG Centre of Expertise Zurich, Universitats-Kinderspital Zurich

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Her presenterer vi en protokoll for å oppdage tumor somatiske mutasjoner i sirkulerende DNA tilstede i pasientens biologiske væsker (biofluids). Vår dråpe Digital polymerase kjedere reaksjon (dPCR)-basert metode muliggjør kvantifisering av tumor mutasjon allel frekvens (MAF), tilrettelegging en minimalt invasiv supplement til diagnostisering og timelig overvåking av tumor respons.

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Kreftforskning flytende biopsi digital dråpe PCR sirkulerende celle fri DNA plasma serum spinalvæske
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