Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Expression, Reinigung, Kristallisation und…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Expression, Reinigung, Kristallisation und Enzym-Assays von Fumarylacetoacetat-Hydrolase-Domänen-haltigen Proteinen
Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
Journal (Biochemistry)
Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
Constructing the Columns and the Mesh System for Leachate Collection to Study Mineral Weathering
Journal (Biochemistry)
Constructing the Columns and the Mesh System for Leachate Collection to Study Mineral Weathering
 
Click here for the English version

Expression, Reinigung, Kristallisation und Enzym-Assays von Fumarylacetoacetat-Hydrolase-Domänen-haltigen Proteinen

Article DOI: 10.3791/59729-v • 10:21 min • June 20th, 2019
June 20th, 2019 •
1,2, 1,2, 1,2, 1, 1, 3, 3, 4, 1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Die Expression und Reinigung von Fumarylacetoacetat-Hydrolase-Domänen-haltigen Proteinen wird anhand von Beispielen beschrieben (Expression in E. coli, FPLC). Gereinigte Proteine werden zur Kristallisation und Antikörperproduktion verwendet und für Enzym-Assays eingesetzt. Ausgewählte fotometrische Assays werden vorgestellt, um die Multifunktionalität von FAHD1 als Oxaloacetat-Decarboxylase und Acylpyruvathydrolase darzustellen.

Tags

Biochemie Ausgabe 148 FAH FAHD FAHD1 ODx ApH Hydrolase Decarboxylase Oxaloacetat Acetylpyruvat Fumarylpyruvat Acylpyruvat Oxalat Pyruvat MiDAS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter