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JoVE Journal Biochemistry
Évaluation des interactions protéines-protéines à l'aide d'une technique de digestion on-Membrane
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Évaluation des interactions protéines-protéines à l'aide d'une technique de digestion on-Membrane

Article DOI: 10.3791/59733-v • 07:07 min • July 19th, 2019
July 19th, 2019 •
*1, *2, 1, 2, 1
1Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 2Department of Biochemistry, Showa University School of Medicine
* These authors contributed equally

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Ici, nous présentons un protocole pour une technique de digestion on-membrane pour la préparation des échantillons pour la spectrométrie de masse. Cette technique facilite l'analyse pratique des interactions protéines-protéines.

Tags

Biochimie Numéro 149 Immunoprécipitation LC/MS/MS protéomique interaction protéique membrane PVDF criblage
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