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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

質量分析による3つの DNA 病変の定量化と、周囲の微細粒子状物質に曝露したマウスの組織におけるそのレベルの評価

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Journal (Immunology and Infection)

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Journal (Immunology and Infection)

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

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質量分析による3つの DNA 病変の定量化と、周囲の微細粒子状物質に曝露したマウスの組織におけるそのレベルの評価

Article DOI: 10.3791/59734-v • 12:15 min • May 29th, 2019

May 29th, 2019 •

Tiago Franco de Oliveira*^1,2, Antonio Anax Falcão de Oliveira*¹, Miriam Lemos³, Mariana Veras³, Paulo Hilário Nascimento Saldiva^3,4, Marisa Helena Gennari de Medeiros⁵, Paolo Di Mascio⁵, Ana Paula de Melo Loureiro¹

¹Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, ²Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, ³Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, ⁴Instituto de Estudos Avançados, Universidade de São Paulo, ⁵Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo

* These authors contributed equally

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Click here for the English version.

ここでは、病変の高感度かつ正確な定量のための方法 8-オキソ-7, 8-ジヒドロ-2'-デオキシグアノシン (8-oxodGuo), 1,n⁶-etheno-2'-デオキシアデノシン (1,n⁶-茶道) および 1,n²-etheno-2'-デオキシグアノシン (1,N₂-dGuo) DNA で.この方法は、曝露された A/J マウスの組織 (肺、肝臓および腎臓) における周囲微細粒子状物質 (PM_2.5) の影響の評価に適用した。

Tags

免疫学および感染症、問題147、質量分析、DNA 付加物、DNA 酸化、遺伝毒性、酸化ストレス、微粒子物質

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