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JoVE Journal
Immunology and Infection

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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
 

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Article DOI: 10.3791/59734-v 12:15 min May 29th, 2019
May 29th, 2019

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Summary

We describe here methods for sensitive and accurate quantification of the lesions 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 1,N6-etheno-2'-deoxyadenosine (1,N6-dAdo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-dGuo) in DNA. The methods were applied to the assessment of the effects of ambient fine particulate matter (PM2.5) in tissues (lung, liver and kidney) of exposed A/J mice.

Tags

DNA Lesions Mass Spectrometry Quantification Oxidative Stress Tissues Mice Ambient Fine Particulate Matter Selectivity Sensitivity HPLC Tandem Mass Spectrometry Etheno Adducts Inflammatory Diseases Pollutants Cancer Development Sample Preparation Culture Plate Tissue Scalpel Weigh
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