JoVE Journal
Immunology and Infection
Immunology and Infection
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环境细颗粒物小鼠组织中三种 DNA 损伤的质谱定量及水平评价
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Article
DOI:
10.3791/59734-v
•
12:15 min
•
May 29th, 2019
环境细颗粒物小鼠组织中三种 DNA 损伤的质谱定量及水平评价
May 29th, 2019
•
Tiago Franco de Oliveira*1,2, Antonio Anax Falcão de Oliveira*1, Miriam Lemos3, Mariana Veras3, Paulo Hilário Nascimento Saldiva3,4, Marisa Helena Gennari de Medeiros5, Paolo Di Mascio5, Ana Paula de Melo Loureiro1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
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我们在这里描述的方法敏感和准确定量的病变 8-氧-7-二氢-2 '-脱氧鸟苷 (8-氧 ',1, n6-乙基 2 '-脱氧腺苷 (1,n6-do)和 1,n2-在 DNA 中的乙二-2 '-脱氧鸟苷 (1,n2-dguo)。应用该方法对暴露的阿可姆小鼠组织 (肺、肝、肾) 环境细颗粒物 (PM2.5) 的影响进行了评价。