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Journal / Immunology and Infection / Kvantificering af tre DNA-læsioner ved…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantificering af tre DNA-læsioner ved massespektrometri og vurdering af deres niveauer i væv af mus udsat for omgivende fine partikler
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Kvantificering af tre DNA-læsioner ved massespektrometri og vurdering af deres niveauer i væv af mus udsat for omgivende fine partikler

Article DOI: 10.3791/59734-v • 12:15 min • May 29th, 2019
May 29th, 2019 •
*1,2, *1, 3, 3, 3,4, 5, 5, 1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
* These authors contributed equally

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Her beskrives metoder til følsom og nøjagtig kvantificering af læsioner 8-oxo-7, 8-dihydro-2'-deoxyguanosin (8-oxodGuo), 1,n6-etheno-2'-deoxyadenosin (1,n6-Dado) og 1,n2- etheno-2'-deoxyguanosin (1,N2-DGUO) i DNA. Metoderne blev anvendt til vurdering af virkningerne af de omgivende fine partikler (PM2,5) i væv (lunge, lever og nyrer) af eksponerede A/J-mus.

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Immunologi og infektion massespektrometri DNA-addukter DNA-oxidation genotoksicitet oxidativ stress fine partikler
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