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JoVE Journal Biochemistry
DNA-sekvens genkendelse af DNA Primase ved hjælp af Primase profilering med høj gennemløb
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Journal (Biochemistry)
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
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DNA-sekvens genkendelse af DNA Primase ved hjælp af Primase profilering med høj gennemløb

Article DOI: 10.3791/59737-v • 08:04 min • October 8th, 2019
October 8th, 2019 •
1, 1, 2, 2, 1, 1
1Department of Chemistry, Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

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Protein binding microarray (PBM) eksperimenter kombineret med biokemiske assays forbinder bindende og katalytiske egenskaber af DNA primase, et enzym, der syntetiserer RNA primere på Template DNA. Denne metode, der er udpeget som et højt gennemløb primase profilering (HTPP), kan bruges til at afsløre DNA-bindende mønstre af en række enzymer.

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Biokemi høj gennemløb primase profilering HTPP proteinbinding microarray PBM DNA primase DNA-replikation protein-DNA interaktion DNA-binding RNA Primers molekylær biologi
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