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Journal / Developmental Biology / Udvikling af målretning mod inducerede lokale…
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Developmental Biology

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JoVE Journal Developmental Biology
Udvikling af målretning mod inducerede lokale læsioner i genomer (TILLING) i små kornafgrøder ved Ethylmethanesulfonat-Mutagenicose
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Udvikling af målretning mod inducerede lokale læsioner i genomer (TILLING) i små kornafgrøder ved Ethylmethanesulfonat-Mutagenicose

Article DOI: 10.3791/59743-v • 08:36 min • July 16th, 2019
July 16th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park

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Beskrevet er en protokol til udvikling af målrettede lokale læsioner i genomer (TILLING) af små kornafgrøder med anvendelse af ethylmethanesulfonat (EMS) som mutagent. Der findes også en protokol til mutation Detection ved hjælp af cel-1-analysen.

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Udviklingsmæssige biologi mutagenese tilling befolkning EMS reverse genetik små kerner cel-1 endonuklease
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