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Immunolabelling myofiber degeneration i muskelbiopsier
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Immunolabelling myofiber degeneration i muskelbiopsier

Article DOI: 10.3791/59754-v • 06:37 min • December 5th, 2019
December 5th, 2019 •
1,2, 1, 1, 1
1Inserm, Institut Mondor de Recherche Biomédicale, 2The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Program, UCL Great Ormond Street Institute of Child Health

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Beskrevet her er en protokol til direkte immun mærkning af nekrotiske myofibers i muskel kryosektioner. Nekrotiske celler er gennemtrængelig for serumproteiner, herunder immunglobulin G (IgG). Afslørende optagelsen af IgG ved myofibers tillader identifikation og kvantificering af myofibers, der gennemgår nekrose uanset muskel tilstand.

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Biologi muskel degeneration myofibre sarcolemma celledød muskel dystrophier nekrose myonecrose nekroptose betændelse immunglobulin
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