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Immunolabelling myofiber degeneration i Muskelbiopsier

Article DOI: 10.3791/59754-v • 06:37 min • December 5th, 2019
December 5th, 2019 •
1,2, 1, 1, 1
1Inserm, Institut Mondor de Recherche Biomédicale, 2The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Program, UCL Great Ormond Street Institute of Child Health

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Beskrivs här är ett protokoll för direkt immunolabelling av nekrotiska myofibers i muskel kryosektioner. Nekrotiska celler är permeabla för serumproteiner, inklusive immunglobulin G (IgG). Avslöjar upptaget av IgG av myofibers möjliggör identifiering och kvantifiering av myofibers som genomgår nekros oavsett muskel tillstånd.

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Biologi muskel degeneration myofibers sarcolemma celldöd muskeldystrophies nekros myonekros necroptos inflammation immunglobulin
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