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Journal / Genetics / Måling af BK-Polyomavirus ikke-kodende kontrol…
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JoVE Journal Genetics
Måling af BK-Polyomavirus ikke-kodende kontrol region drevet Transkriptional aktivitet via flowcytometri
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Måling af BK-Polyomavirus ikke-kodende kontrol region drevet Transkriptional aktivitet via flowcytometri

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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I dette manuskript er en protokol præsenteret for at udføre FACS-baserede måling af BK-Polyomavirus transkriptional aktivitet ved hjælp af HEK293T celler transficeret med en tovejs reporter plasmid udtrykker tdtomato og egfp. Denne metode giver yderligere mulighed for at kvantitativt bestemme indflydelsen af nye forbindelser på viral transkriptionen.

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Genetik BK-Polyomavirus BKPyV ikke-kodning kontrol region NCCR tovejs promotor aktivitet FACS antiviral aktivitet nyretransplantation store T-antigen SV40
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