Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Meting van BK-polyomavirus niet-coderende…
JoVE Journal
Genetics
Author Produced

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Meting van BK-polyomavirus niet-coderende controle regio gedreven transcriptionele activiteit via Flowcytometrie
Author Spotlight: RNAi Inheritance and ChIP in C. elegans
Journal (Genetics)
Author Spotlight: RNAi Inheritance and ChIP in C. elegans
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Journal (Genetics)
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
 
Click here for the English version

Meting van BK-polyomavirus niet-coderende controle regio gedreven transcriptionele activiteit via Flowcytometrie

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

In dit manuscript wordt een protocol gepresenteerd voor het uitvoeren van FACS-based meting van de transcriptionele activiteit van BK-polyomavirus door gebruik te maken van HEK293T cellen met een bidirectionele reporter plasmide die tdTomato en eGFP uitdrukt. Deze methode maakt het mogelijk om de invloed van nieuwe verbindingen op virale transcriptie kwantitatief te bepalen.

Tags

Genetica uitgave 149 BK-polyomavirus BKPyV niet-coderende controle regio NCCR bidirectionele promotor activiteit FACS antivirale activiteit niertransplantatie grote T antigeen SV40
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter