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Måling av BK-polyomavirus ikke-Coding Control region drevet Transcriptional aktivitet via Flow flowcytometri

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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I dette manuskriptet presenteres en protokoll for å utføre FACS-basert måling av BK-polyomavirus transcriptional aktivitet ved å bruke HEK293T celler transfekterte med en toveis reporter plasmider som uttrykker tdTomato og eGFP. Denne metoden videre gjør det mulig å kvantitativt bestemme innflytelsen av romanen forbindelser på viral transkripsjon.

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Genetikk BK-Polyomavirus BKPyV ikke-koding kontroll region NCCR toveis promoter aktivitet FACS antiviral aktivitet nyre transplantasjon store T antigen SV40
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