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Journal / Genetics / Medição de BK-Polyomavirus não-codificação região …
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Medição de BK-Polyomavirus não-codificação região controle driven transcriptional atividade via citometria de fluxo
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Medição de BK-Polyomavirus não-codificação região controle driven transcriptional atividade via citometria de fluxo

Article DOI: 10.3791/59755-v • 11:54 min • July 13th, 2019
July 13th, 2019 •
1,2, 2, 2, 2,3, 3, 3, 4, 1, 2, 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

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Neste manuscrito, um protocolo é apresentado para executar a medida FACS-baseada da atividade transcricional de BK-Polyomavirus usando as pilhas HEK293T transfected com um plasmídeo bidirecional do repórter que expressa tdtomato e EGFP. Este método permite determinar quantitativamente a influência de novos compostos na transcrição viral.

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Genética BK-Polyomavirus BKPyV região de controle não-codificante NCCR atividade promotora bidirecional FACS atividade antiviral transplante renal antígeno T grande SV40
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