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Journal / Genetics / 哺乳动物细胞中基于CRISPR的聚集基因屏幕…
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Genetics

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JoVE Journal Genetics
哺乳动物细胞中基于CRISPR的聚集基因屏幕
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哺乳动物细胞中基于CRISPR的聚集基因屏幕

Article DOI: 10.3791/59780-v • 09:05 min • September 4th, 2019
September 4th, 2019 •
*1, *1, 1, 1, 1,2,3
1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto
* These authors contributed equally

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Summary
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CRISPR-Cas9技术提供了一种有效的方法，可以精确编辑任何细胞类型的哺乳动物基因组，并代表一种执行全基因组基因筛选的新方法。此处提供了一个详细的协议，讨论成功执行池全基因组 CRISPR-Cas9 屏幕所需的步骤。

Tags

遗传学， 问题 151， CRISPR-Cas9， sgRNA 库， 多伦多-敲除， 全基因组CRISPR筛选， 集合辍学屏幕， 功能基因组学， 基本基因， 细胞健康， 增殖
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