Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Schermi genetici basati su CRISPR in cellule…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Schermi genetici basati su CRISPR in cellule mammaliane
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
Journal (Genetics)
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
Journal (Genetics)
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
 
Click here for the English version

Schermi genetici basati su CRISPR in cellule mammaliane

Article DOI: 10.3791/59780-v • 09:05 min • September 4th, 2019
September 4th, 2019 •
*1, *1, 1, 1, 1,2,3
1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

La tecnologia CRISPR-Cas9 fornisce un metodo efficiente per modificare con precisione il genoma dei mammiferi in qualsiasi tipo di cellula e rappresenta un nuovo mezzo per eseguire schermi genetici a livello di genoma. Qui viene fornito un protocollo dettagliato che illustra i passaggi necessari per il successo delle prestazioni degli schermi CRISPR-Cas9 a livello di genoma raggruppato.

Tags

Genetics Numero 151 CRISPR-Cas9 librerie sgRNA Toronto-Knock-out screening CRISPR a livello di genoma schermi di abbandono in pool genomica funzionale geni essenziali fitness cellulare proliferazione
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter