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Samlede CRISPR-baserte genetiske skjermer i pattedyrceller
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Samlede CRISPR-baserte genetiske skjermer i pattedyrceller

Article DOI: 10.3791/59780-v • 09:05 min • September 4th, 2019
September 4th, 2019 •
*1, *1, 1, 1, 1,2,3
1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto
* These authors contributed equally

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CRISPR-Cas9 teknologien skaffer en effektiv metoden å presist redigere det pattedyr Genova dersom noe cellen type og representerer en ny betyr å utføre Genova-bred genetisk skjermene. En detaljert protokoll diskuterer trinnene som kreves for vellykket gjennomføring av samlet Genova hele CRISPR-Cas9 skjermene er gitt her.

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Genetikk CRISPR-Cas9 sgRNA biblioteker Toronto-knock-out Genova-brede CRISPR screening samlede drop-out skjermer funksjonell Genomics essensielle gener celle fitness spredning
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