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Journal / Cancer Research / 使用一套实用可行的方法,描述英国皇家空军家族激酶与疾病相关的突变体…
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JoVE Journal Cancer Research
使用一套实用可行的方法,描述英国皇家空军家族激酶与疾病相关的突变体
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使用一套实用可行的方法,描述英国皇家空军家族激酶与疾病相关的突变体

Article DOI: 10.3791/59795-v • 07:49 min • July 17th, 2019
July 17th, 2019 •
1, 1, 1, 1, 1, 1,2
1The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research, National Cancer Centre Singapore, 2The Cancer and Stem Cell Program, Duke-NUS Medical School

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在本文中,我们提出了一套实用可行的方法,用于描述RAF家族激酶与疾病相关的突变体,包括体外激酶测定、RAF共活化测定和补充裂化荧光酶测定。

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癌症研究,第149期,RAF家族激酶,RAF突变,Ras/RAF/MEK/ERK信号,二分化,二聚体亲和力/稳定性,催化活性,脱模酶活性,体外激酶测定,RAF共激活测定,互补分裂乳色酶测定,癌症
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