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JoVE Journal Cancer Research
Karakterisere sygdomsrelaterede mutanter af RAF-familie kinaser ved hjælp af et sæt praktiske og gennemførlige metoder
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Karakterisere sygdomsrelaterede mutanter af RAF-familie kinaser ved hjælp af et sæt praktiske og gennemførlige metoder

Article DOI: 10.3791/59795-v • 07:49 min • July 17th, 2019
July 17th, 2019 •
1, 1, 1, 1, 1, 1,2
1The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research, National Cancer Centre Singapore, 2The Cancer and Stem Cell Program, Duke-NUS Medical School

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I denne artikel præsenterede vi et sæt praktiske og gennemførlige metoder til karakterisering af sygdomsrelaterede mutanter af RAF-familie kinaser, som omfatter in vitro-kinaseassay, RAF-Co-aktiverings analyse og supplerende split der-analyse.

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Kræftforskning RAF familie kinaser RAF mutation RAS/RAF/Mek/Erk signalering dimerization dimer affinitet/stabilitet katalytisk aktivitet allosterisk aktivitet in vitro kinase assay RAF Co-aktivering assay supplerende split der assay kræft
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