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Journal / Genetics / Transkripsjon Start site kartlegging bruke…
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Genetics

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JoVE Journal Genetics
Transkripsjon Start site kartlegging bruke super-lav input carrier-CAGE
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Transkripsjon Start site kartlegging bruke super-lav input carrier-CAGE

Article DOI: 10.3791/59805-v • 06:59 min • June 26th, 2019
June 26th, 2019 •
1,2, 1,2, 1,2,3
1Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, 2MRC London Institute of Medical Sciences, 3Sars International Centre for Marine Molecular Biology, University of Bergen

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Cap analyse av Gene Expression (CAGE) er en metode for Genova-bred kvantitativ kartlegging av mRNA 5 ' ender å fange RNA polymerase II transkripsjon starte nettsteder på en enkelt-nukleotid oppløsning. Denne arbeide beskriver en lav-input (skive-bur) protokollen for generasjon av høy-kvalitet biblioteker benytter nanogram-beløpene av sum RNA.

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Genetikk cap analyse av Gene Expression lav-input carrier transkripsjon starte nettsteder transkripsjon innvielse nedbrytbart carrier homing endonuclease arrangører enhancers
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