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Journal / Biochemistry / Real-time, semi-automatiseret fluorescerende…
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Biochemistry

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JoVE Journal Biochemistry
Real-time, semi-automatiseret fluorescerende måling af luftvejene overflade flydende pH af primære Human luftvejs epitelial celler
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Real-time, semi-automatiseret fluorescerende måling af luftvejene overflade flydende pH af primære Human luftvejs epitelial celler

Article DOI: 10.3791/59815-v • 10:18 min • June 13th, 2019
June 13th, 2019 •
1, 2,3, 2, 2,4, 1,2, 2,3, 1
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co

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Vi præsenterer en protokol til at foretage dynamiske målinger af luftvejene flydende pH under tynde film betingelser ved hjælp af en plade-læser.

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Biokemi luftveje overflade væske syre-base balance pH plade-læser luft-væske grænseflade luftvejs epithelium cystisk fibrose
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