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JoVE Journal
Biochemistry

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使用新激酶蛋白的强生化方法在分子水平上进行表征
 
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使用新激酶蛋白的强生化方法在分子水平上进行表征

Article DOI: 10.3791/59820-v 11:23 min June 30th, 2019
June 30th, 2019

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Summary

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我们使用强大的生化方法对一种新的激酶蛋白进行了定性:用特殊抗体对不同细胞系和组织进行西带体分析,通过共免疫沉淀实验相互作用,由西方检测到的激酶活性使用磷特异性抗体和由 β+32P+ ATP 标记的斑点。

Tags

生物化学 问题 148 细胞系培养 瞬态转染 免疫沉淀 西布洛特 激酶活性 β=32P+ ATP标签 磷特异性抗体
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