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Journal / Immunology and Infection / Isolering af lamina propria mononukleare celler…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af lamina propria mononukleare celler fra murine Colon ved hjælp af collagenase E
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Isolering af lamina propria mononukleare celler fra murine Colon ved hjælp af collagenase E

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

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Målet med denne protokol er at isolere mononukleare celler, der bor i lamina propria i tyktarmen ved enzymatisk fordøjelse af vævet ved hjælp af collagenase. Denne protokol giver mulighed for effektiv isolering af mononukleare celler, hvilket resulterer i en enkelt cellesuspension, som igen kan bruges til robust immunophenotyping.

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Immunologi og infektion lymfocytter kolon murine lamina propria colitis transplantation flow cytometri enzymatisk fordøjelse inflammation mononukleære celler
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