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Journal / Immunology and Infection / Isolierung von Lamina Propria Mononukleären…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolierung von Lamina Propria Mononukleären Zellen aus murine Colon mit Kollagenase E
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Isolierung von Lamina Propria Mononukleären Zellen aus murine Colon mit Kollagenase E

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

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Das Ziel dieses Protokolls ist es, mononukleäre Zellen, die in der Lamina-Propria des Dickdarms durch enzymatische Verdauung des Gewebes mit Kollagennase leben zu isolieren. Dieses Protokoll ermöglicht die effiziente Isolierung mononukleativer Zellen, was zu einer Einzelzellsuspension führt, die wiederum für eine robuste Immunphenotypisierung verwendet werden kann.

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Immunologie und Infektion Ausgabe 151 Lymphozyten Dickdarm Murin Lamina propria Kolitis Transplantation Durchflusszytometrie enzymatische Verdauung Entzündungen mononukleäre Zellen
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