Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Directe gen knock-out van Axolotl ruggenmerg…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Directe gen knock-out van Axolotl ruggenmerg neurale stamcellen via Elektroporation van CAS9 proteïne-gRNA complexen
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
Journal (Genetics)
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
Journal (Genetics)
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
 
Click here for the English version

Directe gen knock-out van Axolotl ruggenmerg neurale stamcellen via Elektroporation van CAS9 proteïne-gRNA complexen

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier is een protocol voor het uitvoeren van tijd-en ruimtebeperkt gen knock-out in Axolotl ruggengraat koorden door het injecteren van CAS9-gRNA complex in het ruggenmerg centrale kanaal gevolgd door elektroporation.

Tags

Genetica uitgave 149 Axolotl ruggenmerg regeneratie CRISPR Cas9 neurale stamcellen genknock-out
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter