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Journal / Genetics / Direct Gene Knock-out of Axolotl Spinal Cord…
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Genetics

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JoVE Journal Genetics
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
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Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Présenté ici est un protocole pour effectuer le temps et l'espace-limité gène knock-out dans les moelles épinières axolotl en injectant CAS9-gRNA complexe dans le canal central de la moelle épinière suivie d'électroporation.

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Génétique Numéro 149 axolotl moelle épinière régénération CRISPR-Cas9 cellules souches neurales gène knock-out
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