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Journal / Genetics / Direkt Gene knock-out av axolotl ryggmärgen…
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JoVE Journal Genetics
Direkt Gene knock-out av axolotl ryggmärgen neurala stamceller via elektroporation av CAS9 protein-gRNA komplex
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Direkt Gene knock-out av axolotl ryggmärgen neurala stamceller via elektroporation av CAS9 protein-gRNA komplex

Article DOI: 10.3791/59850-v • 09:10 min • July 9th, 2019
July 9th, 2019 •
*1,2, *3, 1, 3, 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
* These authors contributed equally

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Presenteras här är ett protokoll för att utföra tid-och rymdbegränsad Gene knock-out i axolotl ryggmärgen genom att injicera CAS9-gRNA komplex i ryggmärgen Central kanalen följt av elektroporation.

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Genetik axolotl ryggmärg regeneration CRISPR-Cas9 neurala stamceller Gene knock-out
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