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Journal / Cancer Research / Semi-automatisk PD-L1 karakterisering och…
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Cancer Research

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JoVE Journal Cancer Research
Semi-automatisk PD-L1 karakterisering och uppräkning av cirkulerande tumörceller från icke-småcellig Lung cancer patienter genom Immunofluorescensering
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Semi-automatisk PD-L1 karakterisering och uppräkning av cirkulerande tumörceller från icke-småcellig Lung cancer patienter genom Immunofluorescensering

Article DOI: 10.3791/59873-v • 10:29 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 1,2,3, 1,2, 1,2,3, 4, 4, 5, 1, 3, 6,7, 1,2,3
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University

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Summary
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Karakteriseringen av cirkulerande tumörceller (CTCs) är ett populärt ämne i translationell forskning. Detta protokoll beskriver en semi-automatisk immunofluorescensanalys (IF) för PD-L1 karakterisering och uppräkning av CTCs i icke-småcellig lungcancer (NSCLC) patientprover.

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Cancer forskning lungcancer cirkulerande tumörceller cirkulerande fritt DNA immunofluorescenstest CTC spiral mikroflödessystem enhet PD-L1
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