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Journal / Cancer Research / Detection of Retrotransposition Activity of Hot…
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JoVE Journal Cancer Research
Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
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Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Article DOI: 10.3791/59880-v • 10:54 min • July 27th, 2019
July 27th, 2019 •
1,2, 1,2
1Systems Oncology Research Program, University of Helsinki, 2Department of Biochemistry and Developmental Biology, Medicum, University of Helsinki

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Summary
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This article outlines a simple PCR-based assay to monitor the activity of an active LINE-1 retrotransposon and to map de novo retrotranspositions in a given genome. Using the MCF7 cell line, we demonstrate herein how this method can be applied to detect activity of a LINE-1 located at 22q12.1.

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Retrotransposition Activity LINE-1s Long-Distance Inverse PCR Mobile Genetic Elements De Novo Insertions Cancer Genomes Genomic Instability PCR-based Technique High-throughput Sequencing Approaches Biomarker For Malignancy Pre-malignancy Epithelial Tumor Types Colon Cancer High-grade Serous Ovarian Cancer Genomic Aberrations DNA Rearrangements Restriction Enzyme Selection Inverse Primers Design TTC28 LINE-1 Sequence L1Base Database Line-1 Flanking Sequence Polyadenylation Signal Prediction
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