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Journal / Cancer Research / Påvisning af tilbagevirkende aktivitet af Hot…
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Påvisning af tilbagevirkende aktivitet af Hot LINE-1s ved lang distance-inverse PCR
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Påvisning af tilbagevirkende aktivitet af Hot LINE-1s ved lang distance-inverse PCR

Article DOI: 10.3791/59880-v • 10:54 min • July 27th, 2019
July 27th, 2019 •
1,2, 1,2
1Systems Oncology Research Program, University of Helsinki, 2Department of Biochemistry and Developmental Biology, Medicum, University of Helsinki

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Denne artikel skitserer en simpel PCR-baseret analyse til at overvåge aktiviteten af en aktiv linje-1 retroomsætning og til at kort de Novo retrogennem førelser i et givent genom. Ved hjælp af MCF7 Cell line, viser vi heri, hvordan denne metode kan anvendes til at detektere aktivitet af en linje-1 placeret på 22q 12.1.

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Kræftforskning PCR inverse PCR retrotransposons linje-1 retrotransponering strukturel variation kræft DNA mobile DNA genomforskning
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