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Journal / Cancer Research / 长距离反向PCR对热线1的逆变活性的检测…
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Cancer Research
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JoVE Journal Cancer Research
长距离反向PCR对热线1的逆变活性的检测
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Journal (Cancer Research)
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长距离反向PCR对热线1的逆变活性的检测

Article DOI: 10.3791/59880-v • 10:54 min • July 27th, 2019
July 27th, 2019 •
1,2, 1,2
1Systems Oncology Research Program, University of Helsinki, 2Department of Biochemistry and Developmental Biology, Medicum, University of Helsinki

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本文概述了一种基于PCR的简单测定,用于监测活性LINE-1逆转录的活性,并绘制给定基因组中反逆转位图。使用 MCF7 细胞系,我们在此演示如何应用此方法来检测位于 22q12.1 的 LINE-1 的活动。

Tags

癌症研究 问题 149 PCR 反向 PCR 逆型 逆行子 LINE-1 逆转,结构变异 癌症 DNA 移动DNA 基因组学
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