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Journal / Biochemistry / Caenorhabditis elegans中…
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Biochemistry
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JoVE Journal Biochemistry
Caenorhabditis elegans中 2-阿拉希多诺诺基甘油定量的分解标准合成
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Journal (Biochemistry)
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Caenorhabditis elegans中 2-阿拉希多诺诺基甘油定量的分解标准合成

Article DOI: 10.3791/59882-v • 14:25 min • September 21st, 2019
September 21st, 2019 •
1, 2, 2, 2, 1,3
1Instituto de Química Rosario (IQUIR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2Laboratorio de Fisiología Microbiana, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 3Departamento de Química Orgánica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

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这项工作描述了一个强大和直接的方法，以检测和量化内分泌大麻素2-阿拉基诺诺甘醇（2-AG）在C.elegans。我们通过同位素稀释和液相色谱-电喷雾电化-串联质谱（LC-ESI-MS/MS）制备并用于2-AG定量的分析化标准。

Tags

生物化学， 问题 151， 内分泌大麻素， C. elegans，合成， 脱化类比， 2-AG， dauer， MAG， HPLC-MS/MS， 同位素稀释， 定量
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