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JoVE Journal Biochemistry
Sintesi di uno standard deuterato per la quantificazione di 2-Arachidonoylglycerol a Caenorhabditis elegans
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Sintesi di uno standard deuterato per la quantificazione di 2-Arachidonoylglycerol a Caenorhabditis elegans

Article DOI: 10.3791/59882-v • 14:25 min • September 21st, 2019
September 21st, 2019 •
1, 2, 2, 2, 1,3
1Instituto de Química Rosario (IQUIR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2Laboratorio de Fisiología Microbiana, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 3Departamento de Química Orgánica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

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Questo lavoro descrive un metodo robusto e diretto per rilevare e quantificare l'endocannabinoide 2-arachidonoylglycerol (2-AG) in C. elegans. Uno standard analitico deuterato che abbiamo preparato e utilizzato per la quantificazione di 2-AG da diluizione isotopica e cromatografia liquida-elettrospray-spettrometria di massa di mitinazione -tandem (LC-ESI-MS/MS).

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Biochimica Numero 151 endocannabinoidi C. elegans sintesi analoghi deuterati 2-AG dauer MAGs HPLC-MS/MS diluizione isotopica quantificazione
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