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Journal / Biochemistry / Caenorhabditis elegans 2-Arachidonoylglycerol…
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Biochemistry
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JoVE Journal Biochemistry
Caenorhabditis elegans 2-Arachidonoylglycerol Quantification için Bir Deuterated Standart Sentezi
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Caenorhabditis elegans 2-Arachidonoylglycerol Quantification için Bir Deuterated Standart Sentezi

Article DOI: 10.3791/59882-v • 14:25 min • September 21st, 2019
September 21st, 2019 •
1, 2, 2, 2, 1,3
1Instituto de Química Rosario (IQUIR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2Laboratorio de Fisiología Microbiana, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 3Departamento de Química Orgánica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

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Bu çalışma, C. elegansendokannabinoid 2-arachidonoylglycerol (2-AG) tespit etmek ve ölçmek için sağlam ve basit bir yöntem açıklar. 2-AG'nin izotopik seyreltme ve sıvı kromatografi-elektrosprey iyonizasyon-tandem kütle spektrometresi (LC-ESI-MS/MS) ile ölçülmesi için hazırlanmış ve kullanılan analitik bir deuterated standarttır.

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Biyokimya Sayı 151 endkanabinoidler C. elegans sentez deuterated analoglar 2-AG dauer MAGs HPLC-MS/MS izotopik seyreltme nicelleştirme
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