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Journal / Immunology and Infection / 用于病毒感染活成像和定量的路西酶-荧光报告流感病毒…
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Immunology and Infection

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JoVE Journal Immunology and Infection
用于病毒感染活成像和定量的路西酶-荧光报告流感病毒
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用于病毒感染活成像和定量的路西酶-荧光报告流感病毒

Article DOI: 10.3791/59890-v • 05:21 min • August 14th, 2019
August 14th, 2019 •
1, 2, 1,3, 1
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 2Division of Allergy/Immunology and Rheumatology, Department of Medicine, University of Rochester, 3Center for Animal Health Research, INIA-CISA

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甲型流感病毒(IAv)是传染性呼吸道病原体,每年流行和偶尔大流行。在这里,我们描述了一种使用新型重组荧光素酶和荧光表达双报告IAV(BIRFLU)跟踪体内病毒感染的协议。这种方法为研究人员提供了一个极好的工具来研究IAV在体内。

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免疫学和感染 问题 150 流感病毒 荧光 生物发光 复制能力病毒 报告病毒 纳米卢克 Nluc 金星荧光蛋白 病毒感染,体内成像系统 IVIS 小鼠
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