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Immunology and Infection

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JoVE Journal Immunology and Infection
En luciferase-fluorescerende reporter influensa virus for Live Imaging og kvantifisering av viral infeksjon
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En luciferase-fluorescerende reporter influensa virus for Live Imaging og kvantifisering av viral infeksjon

Article DOI: 10.3791/59890-v • 05:21 min • August 14th, 2019
August 14th, 2019 •
1, 2, 1,3, 1
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 2Division of Allergy/Immunology and Rheumatology, Department of Medicine, University of Rochester, 3Center for Animal Health Research, INIA-CISA

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Influensa A virus (IAVs) er smittsom respiratoriske patogener som forårsaker årlige epidemier og sporadisk pandemier. Her beskriver vi en protokoll for å spore virale infeksjoner i vivo ved hjelp av en roman rekombinant luciferase og fluorescens-uttrykker bi-reporter IAV (BIRFLU). Denne tilnærmingen gir forskere et utmerket verktøy for å studere IAV in vivo.

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Immunologi og infeksjon influensa virus fluorescens bioluminesens replikering-kompetente virus reporter virus NanoLuc Nluc Venus fluorescerende protein viral infeksjon in vivo Imaging system IVIS mus
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