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Cancer Research

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JoVE Journal Cancer Research
Onkogen Genfusions detektering ved hjælp af forankret multiplex Polymerasekæde reaktion efterfulgt af næste generations sekvensering
Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia
Journal (Cancer Research)
Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
Journal (Cancer Research)
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Onkogen Genfusions detektering ved hjælp af forankret multiplex Polymerasekæde reaktion efterfulgt af næste generations sekvensering

Article DOI: 10.3791/59895-v • 09:49 min • July 5th, 2019
July 5th, 2019 •
1, 1, 1
1Department of Pathology, University of Colorado - Anschutz Medical Campus

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Denne artikel beskriver brugen af en forankret multiplex polymerase kæde reaktions baseret bibliotek forberedelse kit efterfulgt af næste generation sekventering at vurdere for onkogene gen fusioner i kliniske solide tumorprøver. Både våd-bænk og dataanalyse trin er beskrevet.

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Kræftforskning genfusion præcisionsmedicin Molekylær diagnostik næste generations sekventering forankret multiplex PCR Bioinformatik
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