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Journal / Bioengineering / 用于循环作用电位记录的多井微电极阵列上的人类 iPSC…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
Journal (Bioengineering)
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
Journal (Bioengineering)
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
 
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用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络

Article DOI: 10.3791/59906-v • 08:53 min • July 15th, 2019
July 15th, 2019 •
*1, *1, 1, 1, 1,2,3
1Aurora Research Institute, Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University
* These authors contributed equally

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本文包含一套用于开发人类诱导多能干细胞衍生心肌细胞(hiPSC-CM)网络的协议,这些网络在多孔MEA板上培养,可逆电化细胞膜进行作用电势测量。高通量记录在几天内从同一单元站点重复获取。

Tags

生物工程,第 149 期,iPSC 衍生心肌细胞,多电极阵列,作用电位,场位,心脏电生理学,电穿孔,药物筛选
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