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Journal / Bioengineering / Humane iPSC-afledte Cardiomyocyte-netværk på…
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Bioengineering

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JoVE Journal Bioengineering
Humane iPSC-afledte Cardiomyocyte-netværk på Multiwell Micro-elektrode systemer til tilbagevendende action-potentielle optagelser
Easy and Accurate Mechano-profiling on Micropost Arrays
Journal (Bioengineering)
Easy and Accurate Mechano-profiling on Micropost Arrays
Hybrid µCT-FMT imaging and image analysis
Journal (Bioengineering)
Hybrid µCT-FMT imaging and image analysis
 
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Humane iPSC-afledte Cardiomyocyte-netværk på Multiwell Micro-elektrode systemer til tilbagevendende action-potentielle optagelser

Article DOI: 10.3791/59906-v • 08:53 min • July 15th, 2019
July 15th, 2019 •
*1, *1, 1, 1, 1,2,3
1Aurora Research Institute, Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Denne artikel indeholder et sæt af protokoller for udvikling af menneskeskabte pluripotente stamcelle-afledte cardiomyocyte (hiPSC-CM) netværk dyrket på multiwell MEA plader til reversibelt elektroporat cellemembranen for handling potentielle målinger. Optagelser med høj gennemløb hentes fra de samme celle steder gentagne gange i løbet af dage.

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Bioteknik iPSC-afledte kardiomyocytter multi-elektrode array aktionspotentiale felt potentiale kardiel Elektrofysiologi elektro poration Drug screening
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