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JoVE Journal
Neuroscience

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Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth Ex Vivo
 
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Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth Ex Vivo

Article DOI: 10.3791/59911-v 06:04 min July 16th, 2019
July 16th, 2019

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Summary

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Un présagéex-invivo permet d'imaginer l'excroissance du nerf oculomoteur en temps réel. Les tranches sont générées par l'intégration d'embryons E10.5 IslMN:GFP dans l'agarose, en coupant sur un vibratome et en grandissant dans un incubateur de toit de scène. Le rôle des voies de guidage d'axone est évalué en ajoutant des inhibiteurs aux médias de culture.

Tags

Neurosciences Numéro 149 oculomoteur trouble de dysinnervation crânienne congénitale guidage axone mouvements oculaires imagerie en accéléré culture des tranches
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