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JoVE Journal Genetics
Identification of Footprints of RNA
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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Here, we present a protocol to enrich endogenous RNA binding sites or "footprints" of RNA:protein (RNP) complexes from mammalian cells. This approach involves two immunoprecipitations of RNP subunits and is therefore dubbed RNA immunoprecipitation in tandem (RIPiT).

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RNA Immunoprecipitation RIPiT-Seq RNA Binding Proteins RNA-protein Complexes Sequencing UV-crosslinking Independent Disease-linked RNA Binding Proteins Epitope-tagged Protein Expression CRISPR Tagging Immunoprecipitation Conditions RNase-free Reagents
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