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Journal / Genetics / RNA足迹的识别:通过RNA免疫沉淀在串联中的蛋白质复合物,然后测序(RIPiT-Seq)…
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RNA足迹的识别:通过RNA免疫沉淀在串联中的蛋白质复合物,然后测序(RIPiT-Seq)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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在这里,我们提出一个协议,以丰富内源RNA结合位点或"足迹"的RNA:蛋白质(RNP)复合物从哺乳动物细胞。这种方法涉及RNP亚单位的两个免疫沉淀,因此被称为RNA免疫沉淀串联(RIPiT)。

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遗传学 问题 149 RNA 足迹 RIPiT RNA 结合蛋白 RBP RNA:蛋白质复合物 RNP
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