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JoVE Journal Genetics
Identifiering av spår av RNA
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Identifiering av spår av RNA: protein komplex via RNA immunoprecipitation i tandem följt av sekvensering (RIPiT-SEQ)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Här presenterar vi ett protokoll för att berika endogena RNA bindningsställen eller "fotavtryck" av RNA: protein (RNP) komplex från däggdjursceller. Detta tillvägagångssätt innebär två immunoprecipitations av RNP subenheter och är därför dubbat RNA immunoprecipitation i tandem (ripit).

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Genetik utfärda 149 RNA fotavtryck RIPiT RNA-bindande protein RBP RNA: proteinkomplex RNP
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