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JoVE Journal
Biology

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使用共同分泌的Luciferase代理物测量相对胰岛素分泌
 
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使用共同分泌的Luciferase代理物测量相对胰岛素分泌

Article DOI: 10.3791/59926-v 05:58 min June 25th, 2019
June 25th, 2019

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Summary

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该协议描述了如何使用胰岛素相关的高辛比酶作为β细胞胰岛素分泌的代理,在中等通量下执行快速低成本荧光素酶测定。大多数发光板读取器和多通道移液器都可以进行测定。

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生物学 问题 148 胰岛素 分泌荧光素酶 胰腺β细胞 二氧化二氮范式 分泌测定 高西亚
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