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Måling af relativ insulin sekretion ved hjælp af en co-udskilt luciferase surrogat
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Måling af relativ insulin sekretion ved hjælp af en co-udskilt luciferase surrogat

Article DOI: 10.3791/59926-v • 05:58 min • June 25th, 2019
June 25th, 2019 •
1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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Denne protokol beskriver, hvordan man udfører hurtige lave omkostninger luciferase analyser på medium-gennemløb ved hjælp af en insulin-linked gaussia luciferase som en proxy for insulinsekretion fra beta celler. Analysen kan udføres med de fleste luminescens plade læsere og multikanalspipetter.

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Biologi insulin udskilles luciferase pancreatisk beta celle diazoxid paradigme sekretion assay Gaussia
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